植物乳杆菌(Lactobacillus plantarum)对云龙石斑鱼生长、抗氧化性能及血清生化水平的影响     

李忠琴  邹文政  张光明  杨求华  庄若飞  乔欣君  江兴龙 《海洋与湖沼》2022,53(2):457-465

植物乳杆菌(Lactobacillus plantarum)常作为饲料添加剂,可抑制鱼类病原菌生长,增加机体对饲料的消化和吸收。探讨了饲喂不同添加量的植物乳杆菌对云龙石斑鱼(Epinephelus moara♀×E.lanceolatus♂)生长性能、肠道消化酶活性、肠道和肝脏抗氧化能力、血清生化水平的影响,以期为植物乳杆菌在其健康养殖中的应用提供依据。结果显示,随植物乳杆菌的饲喂量增加,云龙石斑鱼的终末均重(FAW)、增重率(WGR)、特定生长率(SGR)和肠道中淀粉酶(AMS)、脂肪酶(LPS)和糜蛋白酶(CHY)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)的变化均呈现先升高后降低的趋势。而随着植物乳杆菌的饲喂量增加,云龙石斑鱼的血清中总蛋白(TP)、白蛋白(Alb)、胆固醇(TC)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)、丙二醛(MDA)均略降低,甘油三酯(TG)略升高,但与对照组差异不明显(P>0.05)。当添加量为1×10⁵CFU/g时,云龙石斑鱼的各生长指标和抗氧化性能均优于对照组,其中增重率显著提高了19.8%(P<0.05),肠道T-AOC、SOD分别显著升高了93.2%、2.9%(P<0.05),肝脏MDA显著降低了32.7%(P<0.05)。当添加量为1×10⁷CFU/g时,云龙石斑鱼的终末均重、增重率、特定生长率均显著高于对照组和其余添加组(P<0.05);饲料系数显著低于对照组(P<0.05);肠道的AMS、LPS、CHY活性分别显著提高了13.0%、166.9%、61.1%(P<0.05);但肠道和肝脏的抗氧化性能变化不明显,仅肝脏MDA显著降低了23.9%(P<0.05)。由此表明,饲料中添加一定量的植物乳杆菌可以提高肠道消化能力和抗氧化能力,促进云龙石斑鱼的生长,但对其血清生化水平无显著性影响,不会对鱼体代谢产生不良作用。  相似文献   

滇西云龙混合岩化高温热液锡矿床     

王玲  孙杭军 《云南地质》2012,(1):32-35

矿床产于区域性主干断裂旁侧、向斜轴部发育的断裂带中,严格受构造控制。混合岩化花岗岩与锡的形成、富集关系密切,是成矿的母岩。  相似文献   

石斑鱼转录因子NR1D1和NR1D2在冷胁迫中的调控作用          下载免费PDF全文

苗奔奔  林楚  曹文科  梁燕珊  吴仁协  牛素芳  梁镇邦 《应用海洋学学报》2024,43(1):147-159

NR1D1和NR1D2转录因子属于核受体转录因子家族，二者通过调控靶基因表达参与众多生物学过程，尤其在昼夜节律调节中发挥重要作用。本研究通过分析NR1D1和NR1D2的蛋白质分子特征以及在冷胁迫下珍珠龙胆石斑鱼(Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)的基因调控关系以及它们所参与的通路，揭示它们在鱼类低温环境中的功能作用。生物信息学比较分析显示〖STBX〗NR1D1和NR1D2〖ST〗基因序列均有23个开放阅读框，编码的蛋白质均属于不稳定的亲水性蛋白质。NR1D1和NR1D2蛋白质均含有ZF C4类锌脂蛋白结构域和Hormone_Recep核激素受体的配体结合域，主要配体为含铁原卟啉IX。这两种蛋白主要在细胞核中发挥调控基因表达的作用，且与昼夜节律通路的多个关键基因有直接作用关系。结果表明，在冷胁迫下NR1D1和NR1D2作为转录因子可通过调控靶基因的表达，参与昼夜节律调节并促进糖脂代谢、线粒体氧化等过程，为珍珠龙胆石斑鱼提供必要的能量，有助于增强其耐寒性。  相似文献   

Study on the Release of Grouper Epinephelus for Stock Enhancement     

薄治礼  周婉霞 《海洋通报(英文版)》2003,5(1):88-96

Two methods,tagging and ink injection,are used in the grouper releasing experiment.The studied fishes are the juvenile of Epinephelus akaara and Epinephelus awoara both artificially cultivated and caught from natural waters,and their wild adults.Experimental results show that the inhabiting behaviours for both juvenile and adult fishes show distinct regionality,which move within an area of 2n mile diameter 651 days and 48 days after being released,respectively,With the tagging method,the tagged fishes could only be recaptured in the year of release while,with the injection method,they could be caught in the lst,2nd and 3rd years,It is confirmed that in the cement tank,the tagged fishes lose their 1/3 tags.That means that the tagging method is not fit for the release research while the injection method is .Generally,the recaptured rates of injected fishes are 1.4-4.5%,3.1-13.4%and 2.7%for the lst,1nd and 3rd years,respectively.  相似文献   

赤点石斑鱼脑垂体组织生理学的研究   总被引：7，自引：1，他引：7  

方永强 《台湾海峡》1990,9(1):62-72,T001

本文采用多种染色法研究赤点石斑鱼脑垂体各叶不同细胞的形态特征,促性腺激素释放素对嗜碱性细胞的生理影响。讨论了脑垂体促性腺细胞的成熟过程与其性腺发育的关系。  相似文献   

Inhibition of fish nodavirus by gymnemagenol extracted from Gymnema sylvestre     

V.Gopiesh Khanna  K.Kannabiran  V.Sarath Babu  A.S.Sahul Hameed 《中国海洋大学学报(英文版)》2011,10(4):402-408

Viral nervous necrosis(VNN)has emerged to become a major problem in the culture of larval and juvenile marine fish worldwide.Bioactive phytochemicals isolated from commonly available medicinal plants are often screened for their efficacy in controlling fish viral diseases.Occurrence of newer viral strains and resistance to existing antiviral drugs are problems currently as-sociated with treatment of VNN,which necessitates looking for alternate sources for effective antiviral drugs.The aim of the present study was to screen antiviral potential of gymnemagenol(C30H50O4)previously extracted from leaves of Gymnema sylvestre.The fish nodavirus,grouper nervous necrosis virus(GNNV)in infected Sahul Indian Grouper Eye(SIGE)cell lines were used to study the antiviral activity of gymnemagenol under in vitro conditions.The susceptibility of the virus to gymnemagenol was confirmed by measuring the viral titre(TCID50 mL?1)in virus-infected SIGE cells every 24 h.Gymnemagenol at 20 μg mL?1 inhibited the prolifera-tion of GNNV to 53% at the end of the 6th d by inhibiting the proliferation of GNNV-infected SIGE cells.The viable SIGE cells were reduced to 47% as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.The viral titre(TCID50 mL?1)was also reduced to log 2.8 at the end of the 7th d in gymnemagenol-treated SIGE cells after inoculated with GNNV when compared to untreated control SIGE cell viral titre(log 4.1).Based on our results it can be concluded that gymnemagenol could be used as an antiviral agent against GNNV infection.  相似文献   

云南兰坪盆地古近系云龙组上段沉积环境研究——来自碳、氧同位素的证据          下载免费PDF全文

沈立建  刘成林  王立成 《地球学报》2016,37(3):301-306

云南兰坪盆地古近系云龙组地层记录了盆地由淡水湖泊→盐湖、干盐湖→淡水湖泊沉积的一个完整的Ⅰ级韵律。在云龙组上段沉积了一套灰岩地层,上覆于云龙组上段含盐层之上,对此套灰岩共取样25个,测试结果显示,δ~(13)C和δ18O均为负值,δ~(13)C介于–13.13‰~–6.67‰之间,均值为–11.57‰,δ18O介于–15.55‰~–13.90‰之间,均值为–15.02‰,δ~(13)C和δ~(18)O具有较小相关性(0.57),表明当时湖泊封闭性较差。根据Z值和δ~(13)C表明,当时为陆相淡水或微咸水湖泊沉积,含盐度较低。根据经验公式计算所得温度为35.5~44.3℃,平均为41.4℃。相对于现代湖水温度,当时水体温度较高,可能与PETM事件有关。因此,整个云龙组沉积时期气候炎热干旱,造成蒸发岩沉积中断的主要原因为湖泊的封闭性降低。  相似文献   

Using Airgun Source Signals to Study Regional Wave Velocity Changes before and after the Yunlong MS5.0 and Yangbi MS5.1 Earthquakes     

YANG Jianwen  LI Lei  ZHANG Pengying  YE Beng  HE Yingwen  CHEN Ji  CHA Wenjian 《中国地震研究》2019,33(2):320-335

On the basis of the airgun source signals recorded by the stations from January, 2016 to June, 2017, we use cross-correlation detection technology to obtain the characteristics of the stable phase travel time change of each station. We used the Yunlong M_S5.0 and Yangbi M_S5.1 earthquakes as samples. According to regional characteristics, 13 stations with high signal-to-noise ratios and complete data were selected (including 3 fixed stations and 10 active source stations). They are divided into four regions, and on the basis of the GNSS baseline data, the characteristics of regional wave velocity changes before and after the earthquake are analyzed. The results show that the station phase travel time change and the regional stress characteristics represented by the GNSS baseline data have good correlation in the short-term. Due to different degrees of regional stress, there are differences in the travel time changes of different stations in the four regions. Before the Yunlong M_S5.0 and Yangbi M_S5.1 earthquakes, with regional stress adjustment, there is an upward trend in the travel time changes of related stations in the adjacent areas of up to 0.02s. The difference is that there are differences in the time nodes and duration of the travel time anomalies, and there is a reverse descent process after the Yangbi M_S5.1 earthquake. There are different degrees of travel time fluctuations in the relevant stations before and after the two earthquakes, but the fluctuation range before and after the earthquake was small. Compared with the water level change of the reservoir, the adjustment of the regional stress is more likely to have a substantial impact on the travel time changes of the relevant stations.  相似文献   

半胱胺对斜带石斑鱼（Epinephelus coioides）生长激素及其受体mRNA水平的影响          下载免费PDF全文

李云  蔡生力 《海洋与湖沼》2011,42(2):244-250

以海水硬骨鱼类斜带石斑鱼为研究对象,通过投喂和腹腔注射半胱胺盐酸盐（CSH）,研究半胱胺对斜带石斑鱼生长激素（GH）及其受体（GHR）mRNA水平的影响,以初步揭示半胱胺促进斜带石斑鱼生长的作用机理。结果表明,长期投喂CSH可以显著提高斜带石斑鱼的相对体重增长率。投喂和腹腔注射不同剂量的CSH均能明显促进斜带石斑鱼脑垂...  相似文献   

高精度磁测在上坪子铜镍矿区找矿中的应用     

苏昌学  李奇键  燕永锋  范良军  李刚 《云南地质》2012,(1):73-76,65

解释引起上坪子铜镍矿磁异常的地质原因,推断磁异常性质和磁性体埋深,圈定1个找矿靶区和3个异常区,为高精度磁测间接寻找铜镍多金属矿产积累经验。  相似文献